rabbit anti mouse taz antibody  (Novus Biologicals)

Journal: Biology of Reproduction

Article Title: Yes-associated protein 1 is required for proliferation and function of bovine granulosa cells in vitro ^†

doi: 10.1093/biolre/ioz139

Figure Lengend Snippet: Characteristics of antibodies used for western blotting and microscopy.

Article Snippet: Labeling of antibody was verified using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting (data not shown). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody name Dilution ratio Species specificity Source Supplier (distributor, town, and country) Cat. No YAP1 1:400 1 /1:1000 2 /1:200 3 Mouse Rabbit pAB Cell Signaling (Boston, MA, USA) 4912S Phospho-YAP1 (Ser127) 1:400 1 /1:1000 2 /1:200 3 , 4 Mouse Rabbit pAB Cell Signaling 4911S TAZ 1:400 1 /1:1000 2 /1:200 3 Mouse Rabbit pAB Cell Signaling 4883S CYP19A 1:1000 Human Rabbit pAB Abcam (Cambridge, United Kingdom) ab80206 STAR 1:10000 Mouse Rabbit pAB Abcam ab96637 CYP11A1 1:1000 Mouse Rabbit mAB Cell Signaling 14217 HSD3B 1:1000 Mouse Mouse mAB A gift from Dr. Ian Mason TOP2A 1:1000 CalBioChem (Emdmillipore; Burlington, MA, USA) NA14 NFKB1A 1:1000 Mouse Rabbit pAB Abcam ab86299 Cyclin D1 1:1000 Mouse Rabbit mAB Cell Signaling 2978 ACTB 1:5000 Bovine Mouse mAB Sigma Life Science (St. Louis, Missouri, USA) A5441 Alpha-tubulin 1:200 Bovine Mouse mAB Abcam ab7291 HRP-linked 1:10000 Anti-rabbit Jackson ImmunoResearch (West Grove, PA, USA 111035003 HRP-linked 1:10000 Anti-mouse Jackson Laboratory 115035205 DAPI 5 300 nM Thermo Fisher (Carlsbad, CA, USA) D1306 Alexa Fluor 488 1:500 Anti-mouse Invitrogen (Carlsbad, CA, USA) A32723 Alexa Fluor 594 1:500 Anti-rabbit Invitrogen A-11032 Alexa Fluor 647 1:500 Anti-biotin Biolegend (San Diego, CA, USA) 405237 Open in a separate window 1 Diltuion used for immunohistochemistry.

Techniques: Western Blot, Microscopy